| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Highland High School | A | Wright | Wenneker | Keith | Goings | 4:56.53 | Unverified: Knox County |
| 3 | Kirksville High School | A | Herbst | Thomas | Marek | Pigg | 4:39.70 | Verified |
| 4 | Monroe City High School | A | Smith | Chinn | Birt | Williams | 4:25.95 | Unverified: Monroe City Invitational |
| 5 | Palmyra High School | A | Noland | Loman | Sublette | Sheputis | 4:34.57 | Verified |
| 6 | Brookfield High School | A | Wilbeck | Keller | Sharp | Izard | 4:44.65 | Verified |
| 7 | Clark County High School | A | Brunk | St.Clair | Hess | WEAVER | NT | Unverified: |
