| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | WILLIAMS, Emmalee | JR | Monroe City High School | 2:25.27 | Verified |
| 2 | SHARP, Alex | JR | Brookfield High School | 2:25.98 | Verified |
| 3 | CLAY, Nariah | SO | Canton High School | 2:29.00 | Unverified: |
| 4 | SMITH, Lauren | SR | Monroe City High School | 2:34.78 | Unverified: |
| 5 | SHEPUTIS, Laurin | 9 | Palmyra High School | 2:40.00 | Unverified: |
| 6 | JOHNSON, Mady | SR | Clark County High School | 2:41.57 | Verified |
| 7 | TIPTON, Tanner | SR | Green City High School | 2:45.04 | Verified |
| 8 | WRIGHT, Trinity | SO | Highland High School | 2:46.00 | Verified |
| 9 | OBRIEN, Jerorgia | SO | Palmyra High School | 2:47.00 | Unverified: |
| 10 | PIGG, Natalie | SO | Kirksville High School | 2:47.64 | Unverified: |
| 11 | PERKINS, Bella | SR | Palmyra High School | 2:48.72 | Verified |
| 12 | MAREK, Kiersten | SO | Kirksville High School | 2:50.16 | Unverified: |
| 13 | WHEELER, Hannah | SR | Monroe City High School | 2:50.82 | Verified |
| 14 | BREWER, Gabrielle | SO | Kirksville High School | 2:54.95 | Unverified: |
| 15 | HARSHBERGER, Emma | 9 | Highland High School | 2:57.27 | Verified |
| 16 | LUNSFORD, Madison | JR | Green City High School | 2:57.42 | Verified |
| 17 | KESSLER, Madison | 9 | Highland High School | 3:01.22 | Unverified: |
| 18 | JEFFRIES, Britney | 9 | Green City High School | 3:12.66 | Verified |
| 19 | BISHOP, Paige | SO | Scotland County High School | NT | Unverified: |
| 20 | ANDERSON, KENDAL | 9 | Scotland County High School | NT | Unverified: |
| 21 | JOHNSON, Mia | 9 | Clark County High School | NT | Unverified: |
| 22 | WEAVER, MAGGIE | 9 | Clark County High School | NT | Unverified: |
| 23 | ST.CLAIR, Cady | SO | Clark County High School | NT |
