Section 1 of 2
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | EDMONDSON, Aaliyah | 9 | Clark County High School | NT | Unverified: |
| 4 | NEFF, Tiffany | SO | Palmyra High School | 1:05.47 | Verified |
| 5 | NEVES, LUNDYN | 9 | Clark County High School | NT | Unverified: |
| 6 | MILLER, Olivia | SO | Clark County High School | NT |
Section 2 of 2
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BRINGER, Ansley | SO | Highland High School | 56.44 | Verified |
| 2 | KRIGBAUM, Emma | SO | Palmyra High School | 55.21 | Verified |
| 3 | YOUNGBLOOD, Carly | JR | Monroe City High School | 52.62 | Verified |
| 4 | STUFFLEBEAN, Kenslie | SR | Kirksville High School | 48.48 | Verified |
| 5 | BIRT, Lexie | SR | Monroe City High School | 50.55 | Verified |
| 6 | HERBST, Jersey | 9 | Kirksville High School | 53.84 | Verified |
| 7 | KEILHOLZ, Halle | JR | Marion County High School | 55.68 | Verified |
| 8 | GOINGS, Summer | JR | Highland High School | 56.97 | Unverified: |
