Section 1 of 2
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | COUNTRYMAN, Jaylyn | SO | Monroe City High School | 1:09.67 | Verified |
| 2 | PALMISANO, Izick | SR | Keokuk High School | 1:00.99 | Unverified: |
| 3 | WHEELER, CALLAN | 9 | Clark County High School | 59.80 | Unverified: Van Buren Relays |
| 4 | BOGUE, Gideon | 9 | Palmyra High School | 58.55 | Verified |
| 5 | HICKS, Carson | 9 | Palmyra High School | 59.44 | Verified |
| 6 | POLLEY, Trent | JR | Brookfield High School | 1:00.69 | Verified |
| 7 | REAMS, Dakota | JR | Brookfield High School | 1:01.18 | Verified |
Section 2 of 2
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MALLETT, Drew | JR | Highland High School | 56.39 | Verified |
| 2 | BAILEY, Isaiah | SR | Kirksville High School | 55.44 | Verified |
| 3 | DOOSING, Landon | SR | Green City High School | 53.55 | Unverified: |
| 4 | THOMAS, Samuel | SR | Kirksville High School | 51.42 | Verified |
| 5 | BARTO, Christopher | SR | Green City High School | 52.84 | Verified |
| 6 | POTRATZ, Anthony | SR | Keokuk High School | 54.07 | Unverified: |
| 7 | SPURGEON, Aidan | SR | Canton High School | 55.96 | Verified |
| 8 | FULLER, JADIN | 9 | Scotland County High School | 58.00 | Unverified: |
