Section 1 of 2
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Clark County High School | A | Gregory | Poelvoorde | Plenge | Toolate | 1:00.00 | Unverified: Van Buren Relays |
| 4 | Scotland County High School | A | 50.04 | Verified | ||||
| 5 | Kirksville High School | A | Vorkink | Lewis | Nsungula | Lomela | 50.27 | Verified |
Section 2 of 2
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Brookfield High School | A | Baker | Boley | Starlin | Alexander | NT | Verified |
| 3 | Green City High School | A | Fields | Doosing | Barto | Peavler | 46.90 | Unverified: |
| 4 | Highland High School | A | Jones | Job | Goehl | Mallett | 46.44 | Unverified: Knox County |
| 5 | Keokuk High School | A | Robinson | Mcwilliams | Glasgow | Potratz | 46.90 | Unverified: |
| 6 | Monroe City High School | A | Talton | Talton | Jones | Countryman | 47.16 | Verified |
| 7 | Palmyra High School | A | Getz | Juarez | King | Lickfeld | 49.40 | Verified |
