| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Clark County High School | A | Edmondson | Ross | SHAFFER | Pearl | NT | Unverified: |
| 2 | Monroe City High School | A | Freidank | Fortner | Gottman | Pfaff | 59.27 | Unverified: Monroe City Invitational |
| 3 | Brookfield High School | A | Head | Daugherity | Thompson | Izard | 56.26 | Verified |
| 4 | Palmyra High School | A | Hoerr | McBride | Mundy | Sublette | 54.57 | Verified |
| 5 | Highland High School | A | Abell | Bringer | Dreyer | Reed | 55.94 | Unverified: Knox County |
| 6 | Kirksville High School | A | Baumgartner | Hicks | Meyer | Butler | 56.87 | Verified |
| 7 | Scotland County High School | A | Small | ANDERS | Kraus | Bishop | 59.64 | Verified |
