Section 1 of 2
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HEAD, Rylan | JR | Brookfield High School | 31.02 | Verified |
| 2 | WOOD, Clara | 9 | South Shelby High School | 30.21 | Verified |
| 3 | ABELL, Marissa | SR | Highland High School | 29.56 | Unverified: Knox County Relays |
| 4 | THOMPSON, Avery | SR | Brookfield High School | 28.80 | Verified |
| 5 | MITCHELL, Kamryn | 9 | South Shelby High School | 28.95 | Unverified: |
| 6 | LAMPKIN, Alyse | SO | Macon High School | 29.60 | Verified |
| 7 | CLARK, Jaelynn | 9 | Monroe City High School | 30.70 | Unverified: Knox Co. Relays |
| 8 | BREID, Isabelle | 9 | Monroe City High School | NT | Unverified: |
Section 2 of 2
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | ANDERSON, Brooklyn | SO | Macon High School | 28.52 | Verified |
| 2 | PLENGE, Abby | SO | Clark County High School | 27.99 | Unverified: Keokuk Relays |
| 3 | HAWKINS, Carter | SR | Centralia High School | 27.31 | Verified |
| 4 | ELLISON, Alexis | JR | Clark County High School | 25.97 | Verified |
| 5 | WHITE, Taytum | 9 | Palmyra High School | 27.14 | Verified |
| 6 | LEWIS, Shelby | 9 | Centralia High School | 27.44 | Verified |
| 7 | KRIGBAUM, Grace | SR | Palmyra High School | 28.14 | Verified |
| 8 | REED, Alexandria | 9 | Highland High School | 28.58 | Verified |
