| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HAERR, Abe | SR | Palmyra High School | 2:02.15 | Verified |
| 2 | HARLAN, Kabott | JR | Monroe City High School | 2:06.75 | Verified |
| 3 | POLLY, Tyler | SR | Brookfield High School | 2:09.90 | Unverified: Marcus Housman Relays |
| 4 | RIDDLE, Nate | SR | Centralia High School | 2:10.43 | Verified |
| 5 | GRIFFIN, Kaleb | SR | Monroe City High School | 2:17.93 | Verified |
| 6 | JUETTE, Caleb | JR | Palmyra High School | 2:22.64 | Verified |
| 7 | BURKE, Rowan | JR | South Shelby High School | 2:22.87 | Verified |
| 8 | WHEELER, CALLAN | 9 | Clark County High School | 2:24.60 | Verified |
| 9 | GLOVER, Ethan | 9 | Macon High School | 2:24.72 | Verified |
| 10 | LAIR, Sterling | SO | Highland High School | 2:25.18 | Verified |
| 11 | TIMPE, Nathan | SO | Centralia High School | 2:25.31 | Verified |
| 12 | GUILFORD, Caleb | 9 | Brookfield High School | 2:26.54 | Verified |
| 13 | NICHOLS, Colin | JR | Clark County High School | 2:31.39 | Verified |
| 14 | QUINN, Jake | 9 | Macon High School | 2:32.43 | Verified |
| 15 | RUTLEDGE, Colton | SO | Highland High School | 2:34.32 | Verified |
