| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Monroe City High School | A | Freidank | Fortner | Gottman | Clark | 55.99 | Verified |
| 2 | Brookfield High School | A | Daugherity | Head | Thompson | Izard | 54.48 | Verified |
| 3 | Highland High School | A | Dreyer | Reed | Goings | Bringer | 53.44 | Unverified: Knox County Relays |
| 4 | Centralia High School | A | Parrish | Walters | Crum | Bennett | 51.37 | Verified |
| 5 | Palmyra High School | A | Hoerr | Redd | McBride | Sublette | 53.29 | Verified |
| 6 | Macon High School | A | Anderson | Edwards | Richardson | Weimer | 54.24 | Verified |
| 7 | South Shelby High School | A | Gaines | Mitchell | Walker | Wood | 55.22 | Verified |
| 8 | Clark County High School | A | Pearl | Ross | Church | SHAFFER | 59.50 | Verified |
