| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Palmyra High School | A | Bogue | Snyder | Getz | Hicks | 1:47.50 | Verified |
| 2 | South Shelby High School | A | Fenton | Owens | Dodd | Countryman | 1:36.45 | Verified |
| 3 | Centralia High School | A | Greene | Hunter | Erisman | Beard | 1:35.30 | Verified |
| 4 | Highland High School | A | Bringer | Stutsman | Job | Mallett | 1:32.84 | Unverified: Knox County Relays |
| 5 | Monroe City High School | A | Jones | Talton | Martinez | Talton | 1:34.05 | Verified |
| 6 | Macon High School | A | Linear | Riekeberg | McLaughlin | Carpenter | 1:35.88 | Verified |
| 7 | Brookfield High School | A | Abongo | Boley | Polly | Alexander | 1:40.47 | Unverified: Marcus Housman Relays |
| 8 | Clark County High School | A | Gregory | Poelvoorde | Plenge | Toolate | 1:57.03 | Verified |
