| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | PRINDIVILLE, Marie | SR | Nerinx Hall High School | 2:31.32 | Verified |
| 2 | WELCH, Nora | JR | Webster Groves High School | 2:35.31 | Unverified: |
| 3 | STAAB, Sarah | 9 | Webster Groves High School | 2:36.00 | Unverified: |
| 4 | HICKENBOTHAM, Sarah | SR | Kirkwood High School | 2:38.00 | Unverified: |
| 5 | BRADY, Lucy | JR | Nerinx Hall High School | 2:38.92 | Verified |
| 6 | CLANCY, Sophie | SO | Kirkwood High School | 2:48.00 | Unverified: |
| 7 | RISWADKAR, Penelope | 9 | Clayton High School | NT | Unverified: |
| 8 | KIMBERLIN, Mairead | JR | Mehlville High School | NT | Unverified: |
| 9 | LYTLE, Allie | SO | Westminster Christian Academy | NT | Unverified: |
| 10 | HOLOHAN, Sabrina | 9 | Westminster Christian Academy | NT | Unverified: |
| 11 | CHUNG, Rachel | 9 | Clayton High School | NT | Unverified: |
