| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Clayton High School | A | Sanders | Johnson | Blanke | Cuille | NT | Unverified: |
| 2 | McCluer North High School | A | NT | Verified | ||||
| 3 | Kirkwood High School | A | Scully | Chunn | Wilkes | Dixon | 1:50.00 | Unverified: |
| 4 | Webster Groves High School | A | Knapp | Gray | Downey | Bratcher | 1:56.00 | Unverified: |
| 5 | Westminster Christian Academy | A | Bradley | Scheulen | Phillips | Schofield | NT | Unverified: |
| 6 | Mehlville High School | A | Burch | Edwards | Roberts | Pesselato | NT | Unverified: |
