| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Mehlville High School | A | HOORMANN | O'brien | Palmer | TROSKY | NT | Unverified: |
| 2 | Nerinx Hall High School | A | Johnson | Hathaway | MacDonald | Bayer | 5:00.00 | Unverified: time trial |
| 3 | Kirkwood High School | A | Smith | McClure | Pearl | Sebastian | 4:51.50 | Unverified: |
| 4 | Ladue Horton Watkins High School | A | Hanley | Huewe | Hillman | Martin | 4:57.30 | Verified |
| 5 | Webster Groves High School | A | Hepfinger | Juelich | Keegan | Nieman | 5:15.00 | Unverified: |
