| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HILLMAN, Sophia | SO | Ladue Horton Watkins High School | 6:01.00 | Unverified: chaminade Tri meet |
| 2 | DUGGIN, Ava | JR | Kirkwood High School | 6:21.00 | Unverified: |
| 3 | BOLSER, Shelby | SO | Kirkwood High School | 6:21.20 | Unverified: |
| 4 | DREBES, Sarah Ann | SO | Nerinx Hall High School | 6:30.00 | Verified |
| 5 | MAY, Sophia | SO | Ladue Horton Watkins High School | 6:31.00 | Unverified: chaminade Tri meet |
| 6 | HACHMEISTER, Abigail | SR | Pattonville High School | 6:39.00 | Unverified: |
| 7 | JOHNSON, Rose | JR | Webster Groves High School | 6:52.00 | Unverified: |
| 8 | CLIFTON, Taylor | JR | Webster Groves High School | 7:22.00 | Unverified: |
| 9 | BETHELL, Isabelle | SR | Nerinx Hall High School | 7:26.17 | Verified |
| 10 | BERHORST, KATELYN | JR | Mehlville High School | NT | Unverified: |
| 11 | O'BRIEN, Angelina | SR | Mehlville High School | NT | Unverified: |
| 12 | BENNIGHT, Charley | JR | Pattonville High School | NT | Unverified: |
| 13 | GAGNEPAIN, Emily | 9 | Ursuline Academy | NT | Unverified: |
| 14 | FURDEK, Gabriella | 9 | Clayton High School | NT | Unverified: |
| 15 | MITREVA, Ana | SO | Clayton High School | NT | Unverified: |
