| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Mehlville High School | A | Dennis | Roth | TREVISANO | Wiszkon | NT | Unverified: |
| 2 | Kirkwood High School | A | Bert | Perkins | Green | Meyer | 2:05.00 | Unverified: |
| 3 | Pattonville High School | A | Barnes | Jenkins | Rockwell | Daniels | 1:54.19 | Verified |
| 4 | Webster Groves High School | A | Moore | Juelich | Harrison | Logan | 2:03.00 | Unverified: |
| 5 | Nerinx Hall High School | A | Johnson | Padberg | Bayer | Hathaway | 2:30.00 | Unverified: time trial |
| 6 | Ursuline Academy | A | Carapella | Decker | Indelicato | Teng | NT | Unverified: |
