| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MOZINGO, McKenna | JR | Rock Bridge High School | 2:50.00 | Unverified: |
| 2 | KIM, Allison | SO | Rock Bridge High School | 2:58.00 | Unverified: |
| 3 | POWELL, Lilian | 9 | Hickman High School | 3:03.53 | Verified |
| 4 | KEITHAHN, Hazel | 9 | Rock Bridge High School | 3:10.00 | Unverified: |
| 5 | YOUNG, Sabrina | SO | Battle High School | NT | Unverified: |
| 6 | BROADNAX, Amari | SO | Battle High School | NT | Unverified: |
| 7 | HANER, Avery | 9 | Battle High School | NT | Unverified: |
| 8 | HELTIBRAND, Amanda | SO | Hickman High School | NT | Unverified: |
| 9 | ANYE, Sarah | SO | Battle High School | NT | Unverified: |
| 10 | MOSS, Kayla | JR | Battle High School | NT | Unverified: |
| 11 | BYBEE, Etta | 9 | Hickman High School | NT | Unverified: |
| 12 | SAN JUAN RODRIGUEZ, Evelyn | 9 | Battle High School | NT | Unverified: |
| 13 | GOOCH, Emma | 9 | Hickman High School | NT | Unverified: |
| 14 | ASSEFA, Metadel | JR | Battle High School | NT | Unverified: |
