| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Cuba Middle School | A | Smith | Carnal | Greenwalt | Jones | NT | Unverified: |
| 3 | Owensville Middle School | A | Valley | Chapman | Jahnsen | Ely | 5:20.00 | Unverified: |
| 4 | Steelville Middle School | A | Blair | Cottrell | Crocker | Settles | 4:33.23 | Unverified: |
| 5 | Vienna Middle School | A | Auten | Douglas | Zimmer | Rowden | 4:40.00 | Unverified: |
| 6 | St. James Middle School | A | Altis | Marcee | Setzer | Siders | NT | Unverified: |
| 7 | Viburnum Junior High School | A | Rutledge | Rutledge | Oberline | Zundel | NT | Unverified: |
