| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Viburnum Junior High School | A | Barton | Barton | Wigger | Gibbs | NT | Unverified: |
| 2 | St. James Middle School | A | Halbrook | Smith | Campbell | Hughes | NT | Unverified: |
| 3 | Steelville Middle School | A | Collinson | Brame | Quiroz | Edgar | 1:12.11 | Unverified: |
| 4 | Richland Junior High | A | Moss | Bowling | Lercher | Walters | 1:01.80 | Unverified: |
| 5 | Vienna Middle School | A | Parmeley | Schwartze | Davis | Loomis | 1:01.83 | Unverified: |
| 6 | Cuba Middle School | A | Weber | Yates | Ringeison | Silva | NT | Unverified: |
| 7 | Owensville Middle School | A | Winchester | Blankenship | Hinson | Vandegriffe | NT | Unverified: |
