| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | HULSEY, Christian | 9 | Sullivan High School | 1:05.91 | Verified |
| 3 | ANDERSON, Lukas | SO | Sullivan High School | 53.81 | Verified |
| 4 | ZELTMANN, Koen | 9 | St. Francis Borgia High School | 43.68 | Verified |
| 5 | KRAUS, Wyatt | 9 | Sullivan High School | 47.82 | Verified |
| 6 | TOBBEN, Austin | SO | St. Clair High School | 55.46 | Verified |
| 7 | PECHER, Colton | SO | Helias Catholic High School | NT | Unverified: |
