| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | St. Francis Borgia High School | B | 50.00 | Unverified: | ||||
| 3 | Helias Catholic High School | A | Neuenswander | Geisler | Crisostomo | Lanigan | 48.27 | Verified |
| 4 | St. Francis Borgia High School | A | Zeltmann | Volmert | Schumacher | Schmidt | 46.00 | Verified |
| 5 | St. Clair High School | A | Dunn | Sanders | Balderson | Browne | 47.76 | Verified |
| 6 | Helias Catholic High School | B | Knernschield | Melloway | Knox | Malmstrom | 48.27 | Verified |
