| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | GOFF, Aly | SR | SIU Edwardsville | 2:18.50 | |
| 2 | HEAGY, Ashley | Illinois State | 2:19.00 | ||
| 3 | LESHER, Amelia | SO | Bradley | 2:19.84 | Gibson Invitational |
| 4 | JOYCE, Aisling | SR | Bradley | 2:20.50 | |
| 5 | MONSON, Jordan | FR | Saint Louis | 2:22.27 | GVSU Big Meet (Saturday) |
| 6 | HORTON, Gabrielle | FR | SIU Edwardsville | 2:22.50 | |
| 7 | MARCO, Nicole | FR | Saint Louis | 2:23.00 | |
| 8 | KIEFFER, Abby | FR | Saint Louis | 2:31.88 | Mizzou Spring Opener |
| 9 | RUST, Morgan | FR | Saint Louis | NT |
