| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | OWUSU, Pius | SO | Battle High School | 1:16.90 | Verified |
| 2 | BUTEL, Blake | SR | Jefferson City High School | 1:01.65 | Verified |
| 3 | JONES, Jamel | JR | Battle High School | 57.68 | Verified |
| 4 | KAUFFMAN, Kole | SR | Jefferson City High School | 56.87 | Verified |
| 5 | HESTON, Christian | JR | Christian Fellowship School | 57.57 | Verified |
| 6 | SMITH, Benjamin | SO | Rock Bridge High School | 1:00.00 | Unverified: |
| 7 | SWANIGAN, Terrence | JR | Jefferson City High School | 1:03.22 | Verified |
| 8 | SKIRVIN, Haydn | 9 | Hickman High School | NT | Unverified: |
