| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Battle High School | A | Signars | Owusu | Wenzel | Toalson | 3:56.70 | Verified |
| 4 | Jefferson City High School | A | Center | Butel | Myers | Phillips | 3:52.42 | Verified |
| 5 | Jefferson City High School | B | Din-Gabisi | Wright | Smith | Wilson | 3:52.42 | Verified |
| 6 | Rock Bridge High School | A | Colbert | Cooksey | Manuel | Smith | NT | Unverified: |
