| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Battle High School | A | Anthony Gianquinto | Wenzel | Love | Nkongoro | 48.00 | Unverified: COMO vs KC - Battle for the Belt |
| 4 | Hickman High School | A | Ferrao | Harding | Kelley | Zink | 42.86 | Verified |
| 5 | Jefferson City High School | A | Ferguson | Jordan | Dungan | Hood | 48.00 | Unverified: |
