| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Battle High School | A | Carter | Newby | Hopkins | Ferguson | 57.06 | Verified |
| 4 | Hickman High School | A | Baker | Goodman | Leach | Monnig | 55.21 | Unverified: Glendale Girls Night Out |
| 5 | Jefferson City High School | A | Becker | Okpodighe | Hearn | Morris | 55.30 | Unverified: |
