| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | COOKSEY, Ethan | 9 | Rock Bridge High School | 4:58.00 | Verified |
| 2 | COLBERT, Chaz | 9 | Rock Bridge High School | 4:58.00 | Verified |
| 3 | ROUSH, Owen | 9 | Rock Bridge High School | 5:20.00 | Unverified: |
| 4 | IMBODEN, Johnny | SO | Rock Bridge High School | 5:21.00 | Verified |
| 5 | PHILLIPS, Nathan | SR | Jefferson City High School | 5:30.79 | Verified |
| 6 | DIMOND, Parker | SO | Rock Bridge High School | 5:38.93 | Verified |
| 7 | SOSA, Moises | SO | Rock Bridge High School | 5:51.00 | Unverified: |
| 8 | RENNER, Caden | JR | Rock Bridge High School | 5:58.40 | Verified |
| 9 | SCHNAKE, Maddox | 9 | Rock Bridge High School | 6:00.49 | Verified |
| 10 | BOSTER, Lukas | 9 | Rock Bridge High School | 6:05.00 | Unverified: |
| 11 | TICGELAOR, Alex | 9 | Battle High School | 6:24.46 | Verified |
| 12 | TICGELAOR, Ben | 9 | Battle High School | 6:31.27 | Verified |
| 13 | LOCKE, Xavier | 9 | Battle High School | NT | Unverified: |
