| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Battle High School | A | Owusu | Wenzel | Drda | Signars | DQ | Unverified: COMO vs KC - Battle for the Belt |
| 4 | Hickman High School | A | Harding | Kelley | Prewitt | Zink | 1:31.56 | Verified |
| 5 | Jefferson City High School | A | Bishop | Okpodighe | Ferguson | Swanigan | 1:40.07 | Unverified: |
