| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Parkway West High School | A | Watts | Whitaker | Etchason | Sipp | 4:30.58 | Verified |
| 4 | Parkway Central High School | A | Elbert | Tate | Lee | Okpara | 4:09.32 | Verified |
| 5 | Rockwood Summit High School | A | 4:13.19 | Verified |
