| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 99 | SHELTON, Lila | SO | Rockwood Summit High School | 55.04 | Verified |
| 2 | 100 | VINCENT, Avery | JR | Rockwood Summit High School | 53.01 | Verified |
| 3 | 4 | ELBERT, J'nyah | SR | Parkway Central High School | 49.71 | Verified |
| 4 | 98 | BELLAMY, Haley | SR | McCluer North High School | 47.74 | Verified |
| 5 | 6 | PARSONS, Jessica | SR | Parkway Central High School | 49.52 | Verified |
| 6 | 88 | PLASS, Clara | SR | Parkway West High School | 50.26 | Verified |
| 7 | 91 | WATTS, Cecelia | SO | Parkway West High School | 53.78 | Verified |
