| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 2 | 2 | BUFORD, Larickia | SR | McCluer High School | 1:10.62 | Verified |
| 3 | 45 | BOYHER, Julia | JR | Rockwood Summit High School | 1:09.01 | Verified |
| 4 | 33 | OKPARA, Nnenna | JR | Parkway Central High School | 57.42 | Verified |
| 5 | 9 | TURNER, Shea | 9 | Rockwood Summit High School | 1:07.30 | Verified |
| 6 | 46 | HAYEK, Ava | SO | Parkway Central High School | 1:09.74 | Verified |
| 7 | 29 | COOPER, Katrina | SO | McCluer High School | 1:15.00 | Unverified: |
