| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | McCluer North High School | A | 55.23 | Verified | ||||
| 3 | Ladue Horton Watkins High School | A | 54.34 | Verified | ||||
| 4 | Rockwood Summit High School | A | 50.93 | Verified | ||||
| 5 | Parkway Central High School | A | Wangley | Elbert | Jackson | Lee | 53.00 | Unverified: |
| 6 | Parkway West High School | A | Plass | Matthys-Pearce | Woodall | Johnson | 54.80 | Verified |
