| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Rockwood Summit High School | A | 59.00 | Verified | ||||
| 3 | Parkway West High School | A | Thomasson | Williamson | Freed | Malchanava | 54.80 | Verified |
| 4 | Riverview Gardens High School | A | Mccarter | Zimmerman | Shelton | Richardson | 52.25 | Unverified: |
| 5 | Ladue Horton Watkins High School | A | Sopshire | Shafman | Ursery | Schnitzer | DQ | Verified |
| 6 | Parkway Central High School | A | Thuston | Brown | Duffy | Malt | 57.00 | Unverified: |
