| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 75 | SIPP, Emily | SR | Parkway West High School | 5:19.03 | Verified |
| 2 | 58 | WHITAKER, Kennedy | SO | Parkway West High School | 5:34.27 | Verified |
| 4 | 71 | BAIN, Sophia | JR | Parkway Central High School | 5:48.93 | Verified |
| 5 | 74 | REHR, Aryn | JR | Parkway Central High School | 5:49.18 | Verified |
| 6 | 57 | SCHULTE, Virginia | JR | Ladue Horton Watkins High School | NT | |
| 7 | 73 | HINKAMP, Maria | JR | Rockwood Summit High School | 6:13.84 | Verified |
