| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Rockwood Summit High School | A | 1:55.00 | Verified | ||||
| 4 | Riverview Gardens High School | A | Zimmerman | Shelton | Richardson | Mccarter | 1:53.81 | Unverified: |
| 5 | Ladue Horton Watkins High School | A | Busker | Schnitzer | Shafman | Sopshire | 1:54.50 | Unverified: Lafayette Invitational |
| 6 | Parkway Central High School | A | Thuston | Brown | Duffy | Harris | 2:05.00 | Unverified: |
