| Lane | # | Athlete | Team | Seed |
|---|---|---|---|---|
| 1 | 8 | DEJOIE, Lizzie | Parkway Central | 2:12.80 |
| 2 | 9 | HICKEY, Rachel | La Salle-Peru | 2:13.49 |
| 3 | 10 | ROBINSON, Victoria | Lansing | 2:15.71 |
| 4 | 11 | QUANDT, Lainey | Bentonville | 2:16.06 |
| 5 | 12 | VANCARDO, Aly | Lindbergh | 2:16.10 |
| 6 | 13 | DELAHANTY, Brooke | Rosary | 2:16.39 |
| 7 | 14 | PETERSON, Saige | Smith-Cotton Sedalia | 2:17.28 |
| 8 | 15 | WINN, Loral | Dresden | 2:17.47 |
| 9 | 78 | DILLION, Jualianne | Har-Ber | 2:17.91 |