| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Ft. Zumwalt South High School | A | Butler | Pilger | Godefroid | Moore | 1:40.58 | Verified |
| 2 | St. Charles High School | A | Puati | Lopez | Martinez | Wiggs | 1:38.95 | Verified |
| 3 | Francis Howell Central High Scho | A | Gideon | Gil Farias | Glacken | Smith | 1:36.18 | Verified |
| 4 | Troy Buchanan High School | A | Freeman | Hopmann | Keene | Moore | 1:32.65 | Verified |
| 5 | North Point High School | A | Pyatt | Wicklund | Robinson | Trennepohl | NT | Verified |
| 6 | Lutheran (St. Charles) High Scho | A | 1:38.87 | Verified | ||||
| 7 | Ft. Zumwalt East High School | A | Bailey | Jones | Williams | Booker | 1:40.28 | Verified |
| 8 | St. Charles West High School | A | Green | Durham | Politte | Williams | 1:41.52 | Verified |