| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Ft. Zumwalt West High School | A | Entwistle | Rowland | Holland | Lu | NT | Verified |
| 4 | St. Charles West High School | A | Kudaravalli | Scheve | Sprague | Buehrle | 54.66 | Verified |
| 5 | Ft. Zumwalt South High School | A | NT | Verified | ||||
| 6 | Ft. Zumwalt East High School | A | Albers | Albers | Ferguson | Vick | 57.39 | Verified |