| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Soldan International Studies Hig | A | Johnson | Buckner | McRobert | Brock | NT | Unverified: |
| 2 | Ft. Zumwalt East High School | A | Anderson | Wilmes | El Farissi | Borger | 3:39.47 | Verified |
| 3 | Ft. Zumwalt South High School | A | Bates | Beck | Bass | Dewitt | 3:38.69 | Verified |
| 4 | North Point High School | A | Lacke | Trennepohl | Trennepohl | Pyatt | 3:35.24 | Unverified: |
| 5 | St. Charles High School | A | Salamone | Bland | Warner | Nottmeyer | 3:35.30 | Verified |
| 6 | Lutheran (St. Charles) High Scho | A | 3:38.69 | Verified | ||||
| 7 | Troy Buchanan High School | A | Keene | Mahler | Edwards | LaRue | 3:47.95 | Verified |