| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | St. Charles High School | A | Hollrah | Long | Gilkison | O'Keeffe | 4:44.22 | Verified |
| 3 | St. Charles West High School | A | Jones | Myers | Jackson | Jackson | 4:35.06 | Verified |
| 4 | Ft. Zumwalt West High School | A | Anderson | McDaniel | Maupin | Rider | 4:21.08 | Verified |
| 5 | Troy Buchanan High School | A | 4:29.43 | Verified | ||||
| 6 | Francis Howell Central High Scho | A | Halterman | McDevitt | McGowan | Myers | NT | Verified |
| 7 | Ft. Zumwalt South High School | A | NT | Verified |