| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Ft. Zumwalt East High School | A | McClellan | Wilmes | Nair | Borger | 8:20.97 | Verified |
| 2 | St. Charles High School | A | Maples | Reid | Case | Warner | 8:20.98 | Verified |
| 3 | Lutheran (St. Charles) High Scho | A | 8:28.72 | Verified | ||||
| 4 | Francis Howell Central High Scho | A | Casillas-Sanchez | Mehta | Harting | Hamilton | 9:12.77 | Verified |
| 5 | North Point High School | A | Blase | Lerner | Stahl | Simms | 9:20.85 | Verified |
| 6 | St. Charles West High School | A | Kudaravalli | Brasher | Erickson | Reddin | 9:24.42 | Verified |
| 7 | Troy Buchanan High School | A | Wortmann | Victor | Edwards | Gaspariano | 9:28.30 | Verified |
| 8 | Soldan International Studies Hig | A | Brock | Buckner | Fleming | McRobert | NT | Unverified: |