| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | KATAMBWA, Christel | SO | Ft. Zumwalt South High School | 34.01 | Verified |
| 2 | BYRD, Kennedy | JR | Ft. Zumwalt South High School | 31.60 | Verified |
| 3 | KULMA, Charlotte | FR | St. Charles High School | 30.29 | Verified |
| 4 | TZUN-PEREZ, Alison | SO | Ft. Zumwalt West High School | 29.56 | Verified |
| 5 | ADAMS, Alanah | SR | Soldan International Studies Hig | 30.14 | Verified |
| 6 | BELL, Syria | FR | St. Charles High School | 31.58 | Verified |
| 7 | O'KELLEY, Ca'Niah | JR | St. Charles West High School | 33.46 | Verified |
| 8 | BLOCKTON, Jael | FR | Soldan International Studies Hig | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | RIDER, Lyla | SR | Ft. Zumwalt West High School | 28.75 | Verified |
| 2 | LOGAN, Cameryn | SO | Francis Howell Central High Scho | 27.93 | Verified |
| 3 | PETERS, Reagan | FR | Troy Buchanan High School | 27.16 | Verified |
| 4 | NORTH, Elle | SO | Lutheran (St. Charles) High Scho | 26.22 | Verified |
| 5 | ANTHONY, Tijaria | SO | Francis Howell Central High Scho | 26.68 | Verified |
| 6 | HUDSON, Dallyce | JR | Lutheran (St. Charles) High Scho | 27.87 | Verified |
| 7 | ELLISON, Alexis | SR | Troy Buchanan High School | 28.26 | Verified |
| 8 | GIBBS, Destiny | FR | St. Charles West High School | 29.00 | Unverified: |