| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | WALKER, Nakiyah | JR | St. Charles High School | NT | Unverified: |
| 4 | HAYNES, Jocelyn | FR | Troy Buchanan High School | 1:10.18 | Verified |
| 5 | RIDER, Lyla | SR | Ft. Zumwalt West High School | NT |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | FENTON, Lainey | SO | Ft. Zumwalt South High School | 1:09.78 | Verified |
| 2 | PROST, Annabelle | SO | St. Charles West High School | 1:08.00 | Unverified: |
| 3 | HALTERMAN, Hannah | SO | Francis Howell Central High Scho | 1:06.91 | Verified |
| 4 | NORTH, Elle | SO | Lutheran (St. Charles) High Scho | 58.16 | Verified |
| 5 | HARPER, Tess | SO | Ft. Zumwalt South High School | 1:04.96 | Verified |
| 6 | MCDEVITT, Reese | SO | Francis Howell Central High Scho | 1:07.00 | Unverified: |
| 7 | MCDANIEL, Karsyn | SO | Ft. Zumwalt West High School | 1:09.47 | Verified |