| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Ft. Zumwalt East High School | A | Booker | Grimm | Glenn | Terrell | 48.96 | Verified |
| 2 | St. Charles West High School | A | Green | Politte | Williams | Parrish | 46.25 | Verified |
| 3 | Francis Howell Central High Scho | A | Gideon | Gil Farias | Glacken | Smith | NT | Verified |
| 4 | Troy Buchanan High School | A | Freeman | Hopmann | Hall | Moore | 43.74 | Verified |
| 5 | North Point High School | A | Byrd | Robinson | Pyatt | Wicklund | 44.50 | Unverified: |
| 6 | Lutheran (St. Charles) High Scho | A | 46.01 | Verified | ||||
| 7 | Ft. Zumwalt South High School | A | Beck | Butler | Pilger | Godefroid | 46.37 | Verified |
| 8 | St. Charles High School | A | Lopez | Leech | Martinez | Murphy | 53.40 | Verified |