| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Ft. Zumwalt South High School | A | Moller | Krapf | Hendrix | Byrd | 54.73 | Verified |
| 4 | Francis Howell Central High Scho | A | Logan | Johnson | Williams | Anthony | 52.00 | Verified |
| 5 | Troy Buchanan High School | A | Peters | Hunolt | Ellison | Robinson | 52.17 | Verified |
| 6 | Ft. Zumwalt West High School | A | Walden | Tzun-Perez | Gardner | Kuhl | 55.86 | Verified |
| 7 | Lutheran (St. Charles) High Scho | A | NT |