| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 9 | Ft. Zumwalt West High School | A | Linville | McGrath | Anderson | Maupin | 10:14.77 | Verified |
| 10 | Troy Buchanan High School | A | Haynes | Diemer | Witt | Gilbert | 10:51.86 | Verified |
| 11 | Francis Howell Central High Scho | A | Brown | Halterman | McGowan | Breuer | NT | Verified |
| 12 | St. Charles West High School | A | Zeier | Jackson | Wacker | Jackson | 10:59.15 | Verified |
| 13 | St. Charles High School | A | Gilkison | Lauer | Long | O'Keeffe | 11:26.93 | Verified |
| 14 | Ft. Zumwalt South High School | A | Hart | Harper | Pawlik | Holman | 11:44.41 | Verified |