| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Elsberry Middle School | A | Seely | McNelly | Lovelace | Rios | 2:28.07 | Verified |
| 2 | Wright City MS | A | Schulte | Serrano | Speiser | Baugher | 1:54.96 | Verified |
| 3 | Winfield MS | A | Gebert | Mense | Ash | Everly | 1:53.53 | Verified |
| 4 | Black Hawk Middle School | B | 1:50.21 | Verified | ||||
| 5 | Black Hawk Middle School | A | Neeley | Pierce | Tucker | McPike | 1:50.21 | Verified |
| 6 | Winfield MS | B | Keeteman | Meyer | Morgart | Sandholm | 1:53.53 | Verified |
| 7 | North Point Middle School | A | Garrett | Mendoza | Palm | Kuebler | 2:01.04 | Verified |
| 8 | Silex Middle School | A | Hallemeyer | Van Horn | Vehige | Mehler | NT | Unverified: |