| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Winfield MS | A | Amos | Springer | VanHook | Willenbrink | 2:10.40 | Verified |
| 2 | Black Hawk Middle School | A | Lorraine | Bennett | Mitchell | Radar | 2:08.43 | Verified |
| 3 | North Point Middle School | A | Sparkman | Branson | Rice | Bampton | 2:02.08 | Verified |
| 4 | Silex Middle School | A | Freeman | Harris | Kuntz | Harrell | 1:59.55 | Verified |
| 5 | North Point Middle School | B | Hays | Landgraf | Goodson | Foster | 2:02.08 | Verified |
| 6 | Elsberry Middle School | A | Brinkman | Burbridge | Smith | Taylor | 2:06.04 | Verified |
| 7 | Wright City MS | A | Jones | Meier | Wilson | Jones | 2:10.40 | Verified |