| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Winfield MS | B | Meyer | Morgart | Sandholm | Wilson | 4:46.82 | Verified |
| 3 | Black Hawk Middle School | A | Neeley | Gibson | Moreno | Pierce | 4:34.70 | Verified |
| 4 | North Point Middle School | A | Hall | Kmetz | Williams | Prieto | 4:13.44 | Verified |
| 5 | North Point Middle School | B | Gresco | Palm | Hayes | Ball | 4:13.44 | Verified |
| 6 | Winfield MS | A | Davidson | Stokes | Blucker | Cruse | 4:46.82 | Verified |
| 7 | Wright City MS | A | Bricker | Baugher | Johnston | Staats | 4:52.12 | Verified |