| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Wright City MS | A | Goodman | Lopez-Luna | Manner | Ulery | NT | Unverified: |
| 3 | Black Hawk Middle School | A | Lorraine | Bennett | Snyder | Gaffney | 5:13.13 | Verified |
| 4 | North Point Middle School | A | Hunn | Diebel | Swanson | Sparkman | 4:46.52 | Verified |
| 5 | Silex Middle School | A | Claborn | Klein | Lickteig | Schwendemann | 5:08.39 | Verified |
| 6 | Winfield MS | A | Powers | VanHook | Willenbrink | Bray | 5:21.74 | Verified |