| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Elsberry Middle School | A | Montes | Santos | Moore | Ford | NT | Unverified: |
| 2 | Black Hawk Middle School | A | Morris | Lehmkuhl | Neeley | Moreno | 52.88 | Verified |
| 3 | North Point Middle School | A | Hayes | Wilmesherr | Heers | Mozee | 51.68 | Verified |
| 4 | Winfield MS | B | Everly | Keeteman | Stark | Trempler | 51.66 | Verified |
| 5 | Winfield MS | A | Gebert | Galmiche | Mense | Phelps | 51.66 | Verified |
| 6 | Wright City MS | A | Hamel | Speiser | Strunk | Rodriguez | 52.34 | Verified |
| 7 | Black Hawk Middle School | B | 52.88 | Verified |